PUBLICATIONS IN CANCER STEM CELL ENGINEERING
RAPID PROTOTYPABLE BIOMIMETIC PERISTALSIS BIOREACTOR CAPABLE OF CONCURRENT SHEAR AND MULTI-AXIAL STRAIN - PUBMED
January 2022 - Cells, Tissues, Organs
Abigail J Clevenger, Logan Z Crawford, Dillon Noltensmeyer, Hamed Babaei, Samuel B Mabbott, Reza Avazmohammadi, Shreya Raghavan
We engineered a novel rotating screw-drive-based design combined with a peristaltic pump, in order to deliver multiaxial strain and concurrent shear stress to a biocompatible polydimethylsiloxane (PDMS) membrane "wall". The peristalsis bioreactor device has broad utility in the study of development and disease in several organ systems.
PUBLICATIONS IN BIOMATERIALS AND STEM CELL ENGINEERING
THE INFLUENCE OF EXTRACELLULAR MATRIX COMPOSITION ON THE DIFFERENTIATION OF NEURONAL SUBTYPES IN TISSUE ENGINEERED INNERVATED INTESTINAL SMOOTH MUSCLE SHEETS.
August 2014, Biomaterials
Raghavan S, Bitar KN
We describe the formation of tissue engineered innervated sheets, where intestinal smooth muscle and enteric neural stem cells are brought into close association in extracellular matrix (ECM) based microenvironments. Our studies demonstrated that neuronal subtype was modulated by varying ECM composition. This observation could be utilized to derive enriched populations of specific enteric neurons in vitro prior to transplantation.
PUBLICATIONS IN TISSUE ENGINEERING AND REGENERATIVE MEDICINE
PERIANAL IMPLANTATION OF BIOENGINEERED HUMAN INTERNAL ANAL SPHINCTER CONSTRUCTS INTRINSICALLY INNERVATED WITH HUMAN NEURAL PROGENITOR CELLS
Raghavan S, Miyasaka EA, Gilmont RR, Somara S, Teitelbaum DH, Bitar KN
We descibe a regenerative medicine based therapy for fecal incontinence that would directly address the dysfunction of the Internal Anal Sphincter (IAS) muscle. Human IAS tissue constructs were bioengineered from isolated human IAS circular smooth muscle cells and human enteric neuronal progenitor cells. Our results indicate that bioengineered innervated IAS constructs can be used to augment IAS function in an animal model.